منابع مشابه
Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens
The application of forward genetic screens to cultured human cells represents a powerful method to study gene function. The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disrupt gene function in mammalian cells, and has been applied to genome-wide screens. Here, we compare the efficacy of genome-wide CRISPR/Cas9-mediated forward genetic screens versus gene-trap...
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1. Department of Microbiology and Immunology; Diabetes Center at UCSF, WM Keck Center for Noncoding RNAs, University of California, San Francisco, San Francisco, California, USA. 2. Institute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, and Chan-Zuckerberg Biohub, San Francisco, California, USA. 3. Helen Diller Family Compre...
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CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR associated nuclease 9) systems have emerged as versatile and convenient (epi)genome editing tools and have become an important player in medical genetic research. CRISPR-Cas9 and its variants such as catalytically inactivated Cas9 (dead Cas9, dCas9) and scaffold-incorporating single guide sgRNA (scRNA) have been appli...
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The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system for genome editing has greatly expanded the toolbox for mammalian genetics, enabling the rapid generation of isogenic cell lines and mice with modified alleles. Here, we describe a pooled, loss-of-function genetic screening approach suitable for both positive and negative selection that uses a genome-sc...
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ژورنال
عنوان ژورنال: Lab Animal
سال: 2019
ISSN: 0093-7355,1548-4475
DOI: 10.1038/s41684-019-0445-0